![]() ![]() Testing showed it to be very effective in isolating vascular cells. They have named their process vessel isolation and nuclei extraction for sequencing (VINE-seq). After multiple unsuccessful attempts, they found they were able to adapt a method that had been used to isolate splenocytes to isolate vascular cells-and also to assist in cleaning up debris related to death in the brain. They began by using several different types of chemicals to retrieve cells from the basement membrane. The work by the team involved searching for a way to conduct RNA profiling of nuclei from the brain vasculature system. In this new effort, the researchers have found a way around this problem to create a molecular atlas of the brain's vasculature in both a healthy state and where the person who died had Alzheimer's disease. This is because they tend to become lodged in the basement membrane. They note that such a map has not been created due to the difficulty of retrieving cells and nuclei from the brain after a person has died. They suggest the reason for this is the lack of a molecular map of the vasculature of the human brain. As the researchers note, the vasculature system plays a major role in most brain diseases, yet it is still not very well understood. ![]()
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